316 papers
Molecular biology explores the tiny machinery inside living cells, revealing how genetic instructions are read, copied, and turned into the proteins that keep us alive. This field acts as a bridge between the static code of DNA and the dynamic processes that drive growth, disease, and life itself, offering insights into everything from cellular repair to the development of new medicines.
On Gist.Science, we track every new preprint uploaded to bioRxiv in this category to make these complex discoveries accessible to everyone. Our team processes each submission to provide both clear, plain-language explanations and detailed technical summaries, ensuring you can grasp the core findings without getting lost in dense academic jargon.
Below are the latest molecular biology papers freshly processed from bioRxiv, ready for you to explore and understand.
This study demonstrates that inserting an adenosine nucleotide directly upstream of an intrinsic terminator's hairpin significantly enhances protein production and reduces read-through in *E. coli*, a finding validated through systematic engineering and a predictive machine learning model.
This study integrates multi-omics approaches to reveal that the ER-PM contact site protein SYT1 anchors the monolignol biosynthetic complex, thereby optimizing monolignol export for stress-induced lignin deposition.
This study utilizes comparative extracellular proteomics to reveal a nitrogen-responsive secretion profile in *Anabaena*, providing the first evidence of a novel, heterocyst-specific protein export pathway that challenges the classical signal peptide model and offers new avenues for engineering sustainable photosynthetic protein production.
This study demonstrates that the chimeric adenovirus Ad5F35 can efficiently transduce primary human natural killer cells by targeting the CD46 receptor, with transduction efficiency directly correlating to cell-surface CD46 density, which is significantly lower in lymphocytes than in epithelial cells.
The authors developed and validated Tension TRAAKer, a chemigenetic dual-color fluorescent reporter that enables rapid, reversible, and genetically targetable visualization of membrane tension dynamics in living cells by coupling a tension-sensitive TRAAK channel variant with a fluorogenic HaloTag dye.
This study demonstrates that the antiviral drug leritrelvir effectively overcomes resistance to nirmatrelvir by restoring the dimerization of SARS-CoV-2 main protease mutants through tighter binding, thereby maintaining superior inhibitory and cellular antiviral efficacy against resistant variants.
This study demonstrates that perturbing glycosylphosphatidylinositol (GPI)-anchor biosynthesis in *Aspergillus oryzae* alters cell wall composition—specifically by depleting cationic galactosamine and downregulating fusion genes—to induce a dispersed, hyper-branching morphology, offering a novel strategy for optimizing industrial fungal fermentation.
This study introduces a protein-replacement platform combining rapid auxin-inducible degradation with doxycycline-controlled expression to dissect the cellular functions of PABPC, revealing the essential role of the RRM4 domain in growth, functional divergence among paralogs, and the quantitative relationship between PABPC abundance and global poly(A)-tail lengths.
Using integrative MPLEx-based proteomics and metabolomics, this study reveals that nutrient limitation on diluted PDA media reprograms *Colletotrichum sublineola* by downregulating central carbon metabolism and upregulating stress adaptation and conidiation pathways, thereby inducing a starvation-adapted state primed for enhanced sporulation and potential virulence.
This study reports the discovery and characterization of a panel of high-affinity macrocyclic peptides that serve as novel tools for selectively targeting, isolating, and investigating the biology of endogenous HAP40 and its interactions with Huntingtin to advance the understanding of Huntington's disease pathogenesis.